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Wednesday, October 08, 2008

Nobel Laureate: Stanley Prusiner

 

The Nobel Prize in Physiology or Medicine 1997.
"for his discovery of Prions - a new biological principle of infection"


Stanley B. Prusiner (1942 - ) received the Nobel Prize in Physiology or Medicine for demonstrating that the prion protein was responsible for Creutzfeldt-Jakob disease (CJD) in humans and for scrapie in sheep. The significance of this work was that Prusiner rigorously eliminated any trace of RNA and DNA from the preparation of infectious disease particles. He showed for the first time that a protein cold be an infectious agent.

This was a major advance in our understanding of basic biological processes. It led to the discovery that a single protein could adopt two very different folds and one of them could induce folding changes in its neighbors in a catalytic manner. The discovery helps us understand protein folding and chaperones.

THEME:
Nobel Laureates
The press release from the Nobel Prize website does a good job of describing Pusiner's contribution.
6 October 1997

The Nobel Assembly at the Karolinska Institute has today decided to award the Nobel Prize in Physiology or Medicine for 1997 to

Stanley B. Prusiner

for his discovery of "Prions - a new biological principle of infection".

Summary

The 1997 Nobel Prize in Physiology or Medicine is awarded to the American Stanley Prusiner for his pioneering discovery of an entirely new genre of disease-causing agents and the elucidation of the underlying priciples of their mode of action. Stanley Prusiner has added prions to the list of well known infectious agents including bacteria, viruses, fungi and parasites. Prions exist normally as innocuous cellular proteins, however, prions possess an innate capacity to convert their structures into highly stabile conformations that ultimately result in the formation of harmful particles, the causative agents of several deadly brain diseases of the dementia type in humans and animals. Prion diseases may be inherited, laterally transmitted, or occur spontaneously. Regions within diseased brains have a characteristic porous and spongy appearance, evidence of extensive nerve cell death, and affected individuals exhibit neurological symptoms including impaired muscle control, loss of mental acuity, memory loss and insomnia. Stanley Prusiner's discovery provides important insights that may furnish the basis to understand the biological mechanisms underlying other types of dementia-related diseases, for example Alzheimer's disease, and establishes a foundation for drug development and new types of medical treatment strategies.

The prize winning research was initiated 25 years ago

In 1972 Stanley Prusiner began his work after one of his patients died of dementia resulting from Creutzfeldt-Jakob disease (CJD). It had previously been shown that CJD, kuru, and scrapie, a similar disease affecting sheep, could be transmitted through extracts of diseased brains. There were many theories regarding the nature of the infectious agent, including one that postulated that the infectious agent lacked nucleic acid, a sensational hypothesis since at the time all known infectious agents contained the hereditary material DNA or RNA. Prusiner took up the challenge to precisely identify the infectious agent and ten years later in 1982 he and his colleagues successfully produced a preparation derived from diseased hamster brains that contained a single infectious agent. All experimental evidence indicated that the infectious agent was comprised of a single protein, and Prusiner named this protein a prion, an acronym derived from "proteinaceous infectious particle." It should be noted that the scientific community greeted this discovery with great skepticism, however, an unwavering Prusiner continued the arduous task to define the precise nature of this novel infectious agent.

The infectious prion particle forms within the body

Where was the gene encoding the prion, the piece of DNA that determined the sequence of the amino acids comprising the prion protein? Perhaps the gene was closely associated with the protein itself as in a small virus? The answers to these questions came in 1984 when Prusiner and colleagues isolated a gene probe and subsequently showed that the prion gene was found in all animals tested, including man. This startling finding raised even more questions. Could prions really be the causative agent of several dementia-type brain diseases when the gene was endogenous to all mammals? Prusiner must have made a mistake! The solution to this problem became evident with the sensational discovery that the prion protein, designated PrP, could fold into two distinct conformations, one that resulted in disease (scrapie PrP = PrPSc) and the other normal (PrP = PrPc). It was subsequently shown that the disease-causing prion protein had infectious properties and could initiate a chain reaction so that normal PrPc protein is converted into the more stabile PrPSc form. The PrPSc prion protein is extremely stabile and is resistant to proteolysis, organic solvents and high temperatures (even greater than 100o C). With time, non-symptomatic incubation periods vary from months to years, the disease-causing PrPSc can accumulate to levels that result in brain tissue damage. In analogy to a well known literary work, the normal PrPc can be compared to the friendly Dr. Jekyll and the disease causing PrPSc to the dangerous Mr. Hyde, the same entity but in two different manifestations.

Mutations in the prion gene cause hereditary brain diseases

The long incubation time for prion based disease hampered the initial efforts to purify the prion protein. In order to assess purification schemes Prusiner was forced to use scores of mice and in each experiment wait patiently for approximately 200 days for the appearance of disease symptoms. The purification efforts accelerated when it was demonstrated that scrapie could be transferred to hamsters, animals that exhibited markedly shortened incubation times. Together with other scientists, Prusiner cloned the prion gene and demonstrated that the normal prion protein was an ordinary component of white blood cells (lymphocytes) and was found in many other tissues as well. Normal prion proteins are particularly abundant on the surface of nerve cells in the brain. Prusiner found that the hereditary forms of prion diseases, CJD and GSS (see the last section), were due to mutations in the prion gene. Proof that these mutations caused disease was obtained when the mutant genes were introduced into the germline of mice. These transgenic mice came down with a scrapie-like disease. In 1992 prion researchers obtained conclusive evidence for the role of the prion protein in the pathogenesis of brain disease when they managed to abolish the gene encoding the prion protein in mice, creating so called prion knock-out mice. These prion knock-out mice were found to be completely resistant to infection when exposed to disease-causing prion protein preparations. Importantly, when the prion gene was reintroduced into these knock-out mice, they once again became susceptible to infection. Strangely enough, mice lacking the prion gene are apparently healthy, suggesting that the normal prion protein is not an essential protein in mice, its role in the nervous system remains a mystery.
Structural variant disease-causing prions accumulate in different regions of the brain

Specific mutations within the prion gene give rise to structurally variant disease-causing prion proteins. These structural prion variants accumulate in different regions of the brain. Dependent upon the region of the brain that becomes infected, different symptoms, typical for the particular type of disease are evident. When the cerebellum is infected the ability to coordinate body movements declines. Memory and mental acuity are affected if the cerebral cortex is infected. Thalamus specific prions disturb sleep leading to insomnia, and prions infecting the brain stem primarily affect body movement.

Other dementias may have a similar background

Prusiner's pioneering work has opened new avenues for understanding the pathogenesis of more common dementia-type illnesses. For example, there are indications that Alzheimer's disease is caused when certain, non-prion, proteins undergo a conformational change that leads to the formation of harmful deposits or plaques in the brain. Prusiner's work has also established a theoretical basis for the treatment of prion diseases. It may be possible to develop pharmacological agents that prevent the conversion of harmless normal prion proteins to the disease-causing prion conformation.

Intrinsic defense mechanisms do not exist against prions

Prions are much smaller than viruses. The immune response does not react to prions since they are present as natural proteins from birth. They are not poisonous, but rather become deleterious only by converting into a structure that enables disease causing prion proteins to interact with one another forming thread-like structures and aggregates that ultimately destroy nerve cells. The mechanistic basis underlying prion protein aggregation and their cummulative destructive mechanism is still not well understood. In contrast to other infectious agents, prion particles are proteins and lack nucleic acid. The ability to transmit a prion infection from one species to another varies considerably and is dependent upon what is known as a species barrier. This barrier reflects how structurally related the prions of different species are.

Prion diseases in animals and man

Without exception, all known prion diseases lead to the death of those affected. There are, however, great variations in pre-symptomatic incubation times and how aggressively the disease progresses.

Scrapie, a prion disease of sheep, was first documented in Iceland during the 18th century. Scrapie was transferred to Scotland in the 1940s. Similar prion diseases are known to affect other animals, e.g., mink, cats, deer and moose.

Bovine Spongiform Encephalopathy (BSE) - Mad cow disease is a prion disease that has recently received a great deal of publicity. In England BSE was transmitted to cows through feedstuff supplemented with offals from scrapie-infected sheep. The BSE epidemic first became evident in 1985. Due to the long incubation time the epidemic did not peak until 1992. In this year alone roughly 37,000 animals were affected.

Kuru among the Fore-people in New Guinea was studied by Carleton Gajdusek (recipient of the 1976 Nobel Prize in Physiology or Medicine). Kuru was shown to be transmitted in connection with certain cannibalistic rituals and was thought to be due to an unidentified "slow virus". The infectious agent has now been identified as a prion. Duration of illness from first symptoms to death: 3 to 12 months.

Gertsmann-Sträussler-Scheinker (GSS) disease is a hereditary dementia resulting from a mutation in the gene encoding the human prion protein. Approximately 50 families with GSS mutations have been identified. Duration of illness from evidence of first symptoms to death: 2 to 6 years.

Fatal Familial Insomnia (FFI) is due to another mutation in the gene encoding the human prion protein. Nine families have been found that carry the FFI mutation. Duration of illness from evidence of first symptoms to death: roughly one year.

Creutzfeldt-Jakob Disease (CJD) affects about one in a million people. In 85-90% of the cases it has been shown that CJD occurs spontaneously. Ten to fifteen per cent of the CJD cases are caused by mutations in the prion protein gene. In rare instances CJD is the consequence of infection. Previously infections were transmitted through growth hormone preparations prepared from the pituitary gland of infected individuals, or brain membrane transplants. About 100 families are known carriers of CJD mutations. Duration of illness from evidence of first symptoms to death: roughly one year.

A new variant of CJD that may have arisen through BSE-transmission. Since 1995 about 20 patients have been identified that exhibit CJD-like symptoms. Psychological symptoms with depression have dominated, but involuntary muscle contractions and difficulties to walk are also common.


[Photo Credits top: David Powers University of California, San Francisco, bottom: Prionii si bolile prionice]

The 2008 Nobel Prize in Chemistry

 
I'm not a big fan of giving out Nobel Prizes for technological achievements although I do recognize that some of them are noteworthy. This one goes too far in the direction of technology, in my opinion. The technique is useful and has led to many advances in the field but I don't think it's Nobel Prize work.

There were many other worthwhile candidates who made significant advances in the study of basic science, leading to a direct contribution to our understanding of how nature works. None of the names commonly discussed on the science blogs got the prize.

The Chemistry prize was announced today: The Nobel Prize in Chemistry 2008. Here's the press release from the Nobel Prize website.
8 October 2008

The Royal Swedish Academy of Sciences has decided to award the Nobel Prize in Chemistry for 2008 jointly to

Osamu Shimomura, Marine Biological Laboratory (MBL), Woods Hole, MA, USA and Boston University Medical School, MA, USA,

Martin Chalfie, Columbia University, New York, NY, USA

and

Roger Y. Tsien, University of California, San Diego, La Jolla, CA, USA

"for the discovery and development of the green fluorescent protein, GFP".

Glowing proteins – a guiding star for biochemistry

The remarkable brightly glowing green fluorescent protein, GFP, was first observed in the beautiful jellyfish, Aequorea victoria in 1962. Since then, this protein has become one of the most important tools used in contemporary bioscience. With the aid of GFP, researchers have developed ways to watch processes that were previously invisible, such as the development of nerve cells in the brain or how cancer cells spread.

Tens of thousands of different proteins reside in a living organism, controlling important chemical processes in minute detail. If this protein machinery malfunctions, illness and disease often follow. That is why it has been imperative for bioscience to map the role of different proteins in the body.

This year's Nobel Prize in Chemistry rewards the initial discovery of GFP and a series of important developments which have led to its use as a tagging tool in bioscience. By using DNA technology, researchers can now connect GFP to other interesting, but otherwise invisible, proteins. This glowing marker allows them to watch the movements, positions and interactions of the tagged proteins.

Researchers can also follow the fate of various cells with the help of GFP: nerve cell damage during Alzheimer's disease or how insulin-producing beta cells are created in the pancreas of a growing embryo. In one spectacular experiment, researchers succeeded in tagging different nerve cells in the brain of a mouse with a kaleidoscope of colours.

The story behind the discovery of GFP is one with the three Nobel Prize Laureates in the leading roles:

Osamu Shimomura first isolated GFP from the jellyfish Aequorea victoria, which drifts with the currents off the west coast of North America. He discovered that this protein glowed bright green under ultraviolet light.

Martin Chalfie demonstrated the value of GFP as a luminous genetic tag for various biological phenomena. In one of his first experiments, he coloured six individual cells in the transparent roundworm Caenorhabditis elegans with the aid of GFP.

Roger Y. Tsien contributed to our general understanding of how GFP fluoresces. He also extended the colour palette beyond green allowing researchers to give various proteins and cells different colours. This enables scientists to follow several different biological processes at the same time.


[Photo Credit: GFP Glowing Genes]

Tuesday, October 07, 2008

Steve Jones Says Human Evolution Is Over

There's so much wrong with this article by Steve Jones that I don't know where to begin. So I'll leave it up to Sandwalk readers to comment. Steve Jones is a Professor of genetics at University College, London (UK) and the author of Darwin's Ghost.

From Times Online via RichardDawkins.net.
Leading geneticist Steve Jones says human evolution is over
By Julia Belluz


Human evolution is grinding to a halt because of a shortage of older fathers in the West, according to a leading genetics expert.

Fathers over the age of 35 are more likely to pass on mutations, according to Professor Steve Jones, of University College London.

Speaking today at a UCL lecture entitled "Human evolution is over" Professor Jones will argue that there were three components to evolution – natural selection, mutation and random change. "Quite unexpectedly, we have dropped the human mutation rate because of a change in reproductive patterns," Professor Jones told The Times.

"Human social change often changes our genetic future," he said, citing marriage patterns and contraception as examples. Although chemicals and radioactive pollution could alter genetics, one of the most important mutation triggers is advanced age in men.

This is because cell divisions in males increase with age. "Every time there is a cell division, there is a chance of a mistake, a mutation, an error," he said. "For a 29-year old father [the mean age of reproduction in the West] there are around 300 divisions between the sperm that made him and the one he passes on – each one with an opportunity to make mistakes.

"For a 50-year-old father, the figure is well over a thousand. A drop in the number of older fathers will thus have a major effect on the rate of mutation."

Professor Jones added: "In the old days, you would find one powerful man having hundreds of children." He cites the fecund Moulay Ismail of Morocco, who died in the 18th century, and is reputed to have fathered 888 children. To achieve this feat, Ismail is thought to have copulated with an average of about 1.2 women a day over 60 years.

Another factor is the weakening of natural selection. "In ancient times half our children would have died by the age of 20. Now, in the Western world, 98 per cent of them are surviving to 21."

Decreasing randomness is another contributing factor. "Humans are 10,000 times more common than we should be, according to the rules of the animal kingdom, and we have agriculture to thank for that. Without farming, the world population would probably have reached half a million by now – about the size of the population of Glasgow.

"Small populations which are isolated can evolve at random as genes are accidentally lost. World-wide, all populations are becoming connected and the opportunity for random change is dwindling. History is made in bed, but nowadays the beds are getting closer together. We are mixing into a glo-bal mass, and the future is brown."
Be sure to keep in mind the definition of evolution [What Is Evolution?].



My Friend Publishes a Book

 
Many years ago I had a friend who lived just up the street. We didn't get to see each other very much because we went to different high schools. But we did go on one date—to a Simon and Garfunkel concert. It didn't make much of an impression on her because she doesn't even remember it!

Later on she became a well known radio and TV personality including a stint as the co-anchorwoman on the CBC National and a interviewer on "As It Happens" on CBC Radio and NPR.

Now she's written a book and I'm sure it's going to be an excellent read.




Monday, October 06, 2008

Framing Science

 
Matt Nisbet recently posted the following message under the title Framing Science Ranked Among Top 15 Science Blogs.
For the fourth straight month, Framing Science ranks among the top 15 science-related blogs, as tracked by Wikio. The position of a blog in the Wikio ranking depends on the number and weight of the incoming links from other blogs. (Blogrolls are not taken into account and Wikio only counts links from the last 120 days.)
I submitted a comment in which I noted that several famous science blogs such as Bad Astronomy, The Panda's Thumb, and RichardDawkins.net are not included in the Wikio rankings.

Since this has been discussed many times on the blogs, it's safe to assume that Matt knows about it. I think he understands that his ranking among the top 15 blogs is not quite what it seems. So I asked in the comment whether he was deliberately trying to deceive his readers or was this an example of framing?

Unfortunately, my comment must have gone astray in the ether since it didn't survive moderation. Isn't that strange?


Monday's Molecule #91

 
Identify this molecule. You need to describe what you see as accurately as possible and name the species from which this protein was purified. I don't think any of you can do it without a hint but I've received complaints that the hint makes it too easy. We'll see how you do without a hint.1

There's a direct connection between today's molecule and a Nobel Prize. I'm looking for the person(s) who discovered the significance of the molecule—not necessarily the structure.

The first one to correctly identify the molecule and name the Nobel Laureate(s), wins a free lunch at the Faculty Club. Previous winners are ineligible for one month from the time they first collected the prize. There are four ineligible candidates for this week's reward. You know who you are.

THEME:

Nobel Laureates
Send your guess to Sandwalk (sandwalk (at) bioinfo.med.utoronto.ca) and I'll pick the first email message that correctly identifies the molecule and names the Nobel Laureate(s). Note that I'm not going to repeat Nobel Laureate(s) so you might want to check the list of previous Sandwalk postings by clicking on the link in the theme box.

Correct responses will be posted tomorrow. I reserve the right to select multiple winners if several people get it right.

Comments will be blocked for 24 hours. Comments are now open.

UPDATE: The molecule is a fragment of bovine prion protein and the Nobel Laureate is Stanley Prusiner. Four people got it right but the winner is Haruhiko Ishii.


1. OK, maybe one little hint ... this week's Nobel Laureate(s) on Sandwalk were inspired by this week's 2008 winners.

The 2008 Nobel Prize in Physiology or Medicine

 
This one is going to cause a stir in the scientific community. Nobody predicted this [The 2008 Nobel Prize in Physiology or Medicine].

6 October 2008

The Nobel Assembly at Karolinska Institutet has today decided to award The Nobel Prize in Physiology or Medicine for 2008 with one half to

Harald zur Hausen

for his discovery of "human papilloma viruses causing cervical cancer"

and the other half jointly to

Françoise Barré-Sinoussi and Luc Montagnier

for their discovery of "human immunodeficiency virus"

Summary

This year's Nobel Prize awards discoveries of two viruses causing severe human diseases.

Harald zur Hausen went against current dogma and postulated that oncogenic human papilloma virus (HPV) caused cervical cancer, the second most common cancer among women. He realized that HPV-DNA could exist in a non-productive state in the tumours, and should be detectable by specific searches for viral DNA. He found HPV to be a heterogeneous family of viruses. Only some HPV types cause cancer. His discovery has led to characterization of the natural history of HPV infection, an understanding of mechanisms of HPV-induced carcinogenesis and the development of prophylactic vaccines against HPV acquisition.

Françoise Barré-Sinoussi and Luc Montagnier discovered human immunodeficiency virus (HIV). Virus production was identified in lymphocytes from patients with enlarged lymph nodes in early stages of acquired immunodeficiency, and in blood from patients with late stage disease. They characterized this retrovirus as the first known human lentivirus based on its morphological, biochemical and immunological properties. HIV impaired the immune system because of massive virus replication and cell damage to lymphocytes. The discovery was one prerequisite for the current understanding of the biology of the disease and its antiretroviral treatment.

Discovery of human papilloma virus causing cervical cancer

Against the prevailing view during the 1970s, Harald zur Hausen postulated a role for human papilloma virus (HPV) in cervical cancer. He assumed that the tumour cells, if they contained an oncogenic virus, should harbour viral DNA integrated into their genomes. The HPV genes promoting cell proliferation should therefore be detectable by specifically searching tumour cells for such viral DNA. Harald zur Hausen pursued this idea for over 10 years by searching for different HPV types, a search made difficult by the fact that only parts of the viral DNA were integrated into the host genome. He found novel HPV-DNA in cervix cancer biopsies, and thus discovered the new, tumourigenic HPV16 type in 1983. In 1984, he cloned HPV16 and 18 from patients with cervical cancer. The HPV types 16 and 18 were consistently found in about 70% of cervical cancer biopsies throughout the world.

Importance of the HPV discovery

The global public health burden attributable to human papilloma viruses is considerable. More than 5% of all cancers worldwide are caused by persistent infection with this virus. Infection by the human papilloma virus is the most common sexually transmitted agent, afflicting 50-80% of the population. Of the more than 100 HPV types known, about 40 infect the genital tract, and 15 of these put women at high risk for cervical cancer. In addition, HPV is found in some vulval, penile, oral and other cancers. Human papilloma virus can be detected in 99.7% of women with histologically confirmed cervical cancer, affecting some 500,000 women per year.

Harald zur Hausen demonstrated novel properties of HPV that have led to an understanding of mechanisms for papilloma virus-induced carcinogenesis and the predisposing factors for viral persistence and cellular transformation. He made HPV16 and 18 available to the scientific community. Vaccines were ultimately developed that provide ≥95 % protection from infection by the high risk HPV16 and 18 types. The vaccines may also reduce the need for surgery and the global burden of cervical cancer.

Discovery of HIV

Following medical reports of a novel immunodeficiency syndrome in 1981, the search for a causative agent was on. Françoise Barré-Sinoussi and Luc Montagnier isolated and cultured lymph node cells from patients that had swollen lymph nodes characteristic of the early stage of acquired immune deficiency. They detected activity of the retroviral enzyme reverse transcriptase, a direct sign of retrovirus replication. They also found retroviral particles budding from the infected cells. Isolated virus infected and killed lymphocytes from both diseased and healthy donors, and reacted with antibodies from infected patients. In contrast to previously characterized human oncogenic retroviruses, the novel retrovirus they had discovered, now known as human immunodeficiency virus (HIV), did not induce uncontrolled cell growth. Instead, the virus required cell activation for replication and mediated cell fusion of T lymphocytes. This partly explained how HIV impairs the immune system since the T cells are essential for immune defence. By 1984, Barré-Sinoussi and Montagnier had obtained several isolates of the novel human retrovirus, which they identified as a lentivirus, from sexually infected individuals, haemophiliacs, mother to infant transmissions and transfused patients. The significance of their achievements should be viewed in the context of a global ubiquitous epidemic affecting close to 1% of the population.

Importance of the HIV discovery

Soon after the discovery of the virus, several groups contributed to the definitive demonstration of HIV as the cause of acquired human immunodeficiency syndrome (AIDS). Barré-Sinoussi and Montagnier's discovery made rapid cloning of the HIV-1 genome possible. This has allowed identification of important details in its replication cycle and how the virus interacts with its host. Furthermore, it led to development of methods to diagnose infected patients and to screen blood
products, which has limited the spread of the pandemic. The unprecedented development of several classes of new antiviral drugs is also a result of knowledge of the details of the viral replication cycle. The combination of prevention and treatment has substantially decreased spread of the disease and dramatically increased life expectancy among treated patients. The cloning of HIV enabled studies of its origin and evolution. The virus was probably passed to humans from chimpanzees in West Africa early in the 20th century, but it is still unclear why the epidemic spread so dramatically from 1970 and onwards.

Identification of virus−host interactions has provided information on how HIV evades the host’s immune system by impairing lymphocyte function, by constantly changing and by hiding its genome in the host lymphocyte DNA, making its eradication in the infected host difficult even after long-term antiviral treatment. Extensive knowledge about these unique viral host interactions has, however, generated results that can provide ideas for future vaccine development as well as for therapeutic approaches targeting viral latency.

HIV has generated a novel pandemic. Never before has science and medicine been so quick to discover, identify the origin and provide treatment for a new disease entity. Successful anti-retroviral therapy results in life expectancies for persons with HIV infection now reaching levels similar to those of uninfected people.

Harald zur Hausen, born 1936 in Germany, German citizen, MD at University of Düsseldorf, Germany. Professor emeritus and former Chairman and Scientific Director, German Cancer Research Centre, Heidelberg, Germany.

Françoise Barré-Sinoussi, born 1947 in France, French citizen, PhD in virology, Institut Pasteur, Garches, France. Professor and Director, Regulation of Retroviral Infections Unit, Virology Department, Institut Pasteur, Paris, France.

Luc Montagnier, born 1932 in France, French citizen, PhD in virology, University of Paris, Paris, France. Professor emeritus and Director, World Foundation for AIDS Research and Prevention, Paris, France.



Friday, October 03, 2008

Obama, Biden, McCain and Palin Agree on One Thing

 
Same-sex marriage is the law of the land in Canada and in many (most?) other Western industrialized nations. It's against the law in most states in the USA.

I was aware of the fact that John McCain and Sarah Palin were opposed to legalization of same-sex marriage. Last night I was shocked to learn that Barack Obama and Joe Biden also oppose legalization of same-sex marriage.

Unless the following is incorrect ...
IFILL: Let's try to avoid nuance, Senator. Do you support gay marriage?

BIDEN: No. Barack Obama nor I support redefining from a civil side what constitutes marriage. We do not support that. That is basically the decision to be able to be able to be left to faiths and people who practice their faiths the determination what you call it.

The bottom line though is, and I'm glad to hear the governor, I take her at her word, obviously, that she think there should be no civil rights distinction, none whatsoever, between a committed gay couple and a committed heterosexual couple. If that's the case, we really don't have a difference.

IFILL: Is that what your said?

PALIN: Your question to him was whether he supported gay marriage and my answer is the same as his and it is that I do not.

IFILL: Wonderful. You agree. On that note, let's move to foreign policy.
Isn't this the 21st century? Isn't Obama supposed to be a progressive?


Vote

 
There's an election coming up in Canada and the USA. Canada's is first.

No matter what you think about the candidates it's important to go out and vote. I plan on voting several times at least once for somebody. I don't know who, yet.

This is a really, really cool video made by a bunch of people you may recognize. Even if you've already voted in the advance polls you should watch this video. It will make you laugh (unless you're offended by four letter words and women removing their bras).




[Hat Tip: Phil Plait of Bad Astronomy]

Tangled Bank #115

 
The latest issue of Tangled Bank has been published on Evolved and Rational [The Tangled Bank #115: The awesome level is over 9000!].
Welcome to the 115th edition of the Tangled Bank, a biweekly blog carnival featuring the best science and medicine posts in the blogosphere. The name of this carnival was taken from Charles Darwin's famous metaphor:
"It is interesting to contemplate a tangled bank, clothed with many plants of many kinds, with birds singing on the bushes, with various insects flitting about, and with worms crawling through the damp earth, and to reflect that these elaborately constructed forms, so different from each other, and dependent upon each other in so complex a manner, have all been produced by laws acting around us."
If you are new to Evolved and Rational, this blog deals with science, evolution, creationism, skepticism, atheism, and internet culture; albeit with a generous serving of lulz. If you like what you see, please subscribe to the RSS feed.


Send an email message to host@tangledbank.net if you want to submit an article to Tangled Bank. Be sure to include the words "Tangled Bank" in the subject line. Remember that this carnival only accepts one submission per week from each blogger.

Thursday, October 02, 2008

Armor of God Pajamas

 

Hands up all of you who think that these armor pajamas are going to make the kids feel safe and secure at night?

I didn't think so.

The pajamas alone simply won't do. They'll also need semi-automatic handguns.


[Hat Tip: Friendly Atheist]

Any Questions?

 
David Scott Springer (DaveScot) is one of the IDiots who post on the Intelligent Design Creationism websites. One of his latest is an example of Google Trends, a nifty way of tracking Google search terms over a period of five years.

DaveScot posted a graph on Uncommon Descent showing the trend for "Intelligent Design" (blue), "Darwinian Evolution" (red), "Scientific Creationism" (orange), and "Theological Evolution" (green) [Fun With Google Trends - ID vs. Darwinism vs. Creationism]. Here it is ...


He ended the posting with the enigmatic "Any questions?"

Yes, as a matter of fact, I have many questions. For starters, why is there only a temporary blip in "Intelligent Design" in the Fall of 2005? That's when the Dover trial was in full swing so you might expect there to be an upswing in interest as the trial went on. After all, the daily reports were highlighting the destruction of Intelligent Design as a credible phenomenon and everyone likes a train wreck.

But why was there no significant interest before 2005 or after?

And why did he use "Darwinian evolution" as his query when he knows full well that this is mostly a creationist term.(Update) The only people searching for articles on "Darwinian evolution" would be creationists. Wouldn't it be more informative to find out who was interested in just plain "evolution?" Wouldn't it be fun to see if that search term outranked "Intelligent Design?" You bet.

So here's the result: the top graph is for the USA and the bottom one is for the United Kingdom.




Any questions? I didn't think so.

Those of us who are involved in the creation/evolution debate tend to forget how little the general public knows about Intelligent Design Creationism. I'm teaching a class on scientific controversies and one section is about the evolution/creation debate. The 50 students in my class probably are there, in part, because they have an interest in this debate. When I asked them to explain "Intelligent Design" only a handful (~5) had any idea what it was and most of the students claimed they had never heard of it.

It looks like the wedge strategy isn't working very well.

Update: DaveScot was asked on Uncommon Descent why he used the term "Darwinian evolution" instead of just "evolution." His reply? .... "ID doesn’t dispute all “evolution”. It disputes Darwinian evolution." Is anyone still wondering why we call them IDiots?


Wednesday, October 01, 2008

Walking with Charles Darwin

 
Stavros Isaiadis posted this photograph of Darwin's walking stick on his blog Journey Through a Burning Mind [Damn those Immoral Darwinists!].



I'm sure Darwin used it while strolling on the Sandwalk. Undoubtedly the skull reminded him of his evil plan to destroy religion by publishing a book about evolution.



The New Model Organisms

 
A model organism is one that is amenable to a variety of studies. It often means that it has a well established genetics and that it is relatively easy to maintain in the laboratory.

Back in the late 1960s and early 1970s, scientists who were part of the 'phage group began to look around for new model organisms—especially eukaryotes. I decided to move from bacteriophage T4 to an already existing model organism, Drosophila melanogaster. Some workers set up entirely new systems, such as Caenorhabditis elegans [Nobel Laureates: Sydney Brenner, Robert Horvitz, John Sulston].

The research scientists who I knew at the time were having fierce debates over the proper choice of a new model organism and some of them choose systems that did not pan out. The ones that caught on were species like mouse, human, Arabidopsis, Tetrahymena, Dictyostelium, zebrafish, and some stange little fungus called Saccharomyces cerevisiae.

Now there's a whole new group of model organisms on the market and Cold Spring Harbor Laboratory has decided to publish protocols for each one of them [Emerging Model Organisms].

Here's the complete list. Some of them are new to me.

* Nematode (Pristionchus pacificus)
* Opossum (Monodelphus domestica)
* Planarians
* Snapdragon (Antirrhinum)
* Spider (Cupennius salei)
* Amphipod (Parhyale hawaiensis)
* Bichirs (Polypterus)
* Blind Cave Fish (Astyanax mexicanus)
* Butterfly (Bicyclus anynana)
* Choanoflagellates
* Comb Jellies (Ctenophora)
* Cricket (Gryllus bimaculatus)
* Demosponge (Amphimedon queenslandica)
* Dogfish (Scyliorhinus canicula)
* Finches
* Fruit Bat (Carollia perspicillata)
* Lamprey (Petromyzon marinus)
* Leech (Helobdella)
* Moss (Physcomitrella patens)
* Quail (Coturnix coturnix japonica)
* Snail (Ilyanassa obsoleta)
* Social Ameba (Dictyostelium discoideum)
* Tomato (Solanum lycopersicum)


Nobel Laureate: Severo Ochoa

 

The Nobel Prize in Physiology or Medicine 1959.
"for their discovery of the mechanisms in the biological synthesis of ribonucleic acid and deoxyribonucleic acid"

Severo Ochoa (1905 -1993 ) received the Nobel Prize in Physiology or Medicine for his discovery of an enzyme that synthesizes RNA from ribonucleotide triphosphates. The enzyme, polynucleotide phosphorylase [Monday's Molecule #90], was first isolated from the bacterium Azotobacter vinelandii. At the time it was thought to be the enzyme responsible for the synthesis of RNA in cells as described in the excerpt from the presentation speech below from the Nobel Prize website [1959 Presentation Speech].

Ochoa shared his prize with Arthur Kornberg who discovered a DNA polymerase [Nobel Laureate: Arthur Kornberg].

THEME:
Nobel Laureates
Ochoa's enzyme produces ribonucleic acids from ribonucleotides having twice the ratio of phosphoric acid residues as that contained in ribonucleic acid. The ribonucleic acid is formed by splitting out half of the phosphoric acid residues, and linking the nucleotides together to form large molecules, which, as far as we can prove today, do not differ in any way from natural nucleic acids. Kornberg's enzyme produces deoxyribonucleic acids in a similar, but not identical fashion. Both have arrived at the same, principally important result that in order to make the reaction start, it is necessary to add in the beginning a small amount of nucleic acid to act as a template. Otherwise the enzymes do not «know» which kind of nucleic acid they are to produce. As soon as they get a template to act as a guide, they start, just like a skilled type-setter, to copy the «manuscript» they have received. Here one recognizes life's own principle that like creates like. Even though several research workers had earlier suspected that such a mechanism was involved, the actual experimental proof is of greatest importance. Furthermore, Ochoa's enzyme has given us the possibility of enzymatically synthesizing simplified nucleic acids of great interest
Within a short time, scientists began to realize that polynucleotide phosphorylase did not require a DNA template. The enzyme synthesized random polymers of ribonucleotides in a reaction that is now recognized as a way of salvaging RNA in bacterial cells.

Within a few years Marshall Nirenberg, Gobind Khorana, and others began to exploit this enzyme to synthesize synthetic RNAs that were used to crack the genetic code [Nobel Laureates: Robert W. Holley, Har Gobind Khorana, and Marshall W. Nirenberg].

This is one example of a Nobel Prize that was awarded for the wrong reasons but few people begrudge Ochoa since he was widely recognized as an outstanding scientist. Arthur Kornberg worked with him for one year (1946) in New York where he (Kornberg) purified his first enzymes and fell in love with enzymes (Kornberg, 2001).

Ochoa was born in Spain and graduated from the University of Madrid with an M.D. degree in about 1925. He worked with Otto Meyerhof [Nobel Laureates: Otto Fritz Meyerhof] for a few years on metabolic enzymes before joining a series of labs in Europe. Eventually the civil war in Spain and the outbreak of World War II in Europe led him to join Carl and Gerty Cori in St. Louis, Missouri (USA) in 1942 [Nobel Laureates: Carl Ferdinand Cori and Gerty Theresa Cori]. From there he moved to New York University School of Medicine where he remained until his retirement in 1974.

Ochoa did not return to Spain until the year Franco died (1975). He celebrated his 70th birthday in spectacular manner according to Arthur Kornberg (Kornberg, 2001).
To celebrate his 70th birthday in 1975, Ochoa chose as guests the scientists he most respected worldwide. Symposia and celebratory dinners, starting in Barcelona, were followed by a visit with Salvador Dali in his museum in his hometown in Figueras and culminated in a gala of events in Madrid. It was a party, the likes of which has not been seen in scientific circles before or since.


[Photo Credits: Top: Kornberg (2001), Bottom: Severo Ochoa: La Conquista del Nobel]

Kornberg, A. (2001) Remembering Our Teachers. J. Biol. Chem. 276:3-11. [JBC Online]